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Berthold Detection Systems orion ii microplate luminometer
Orion Ii Microplate Luminometer, supplied by Berthold Detection Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/orion+ii+microplate+luminometer/sirius+luminometer/pm40604246-79-7-11
Average 90 stars, based on 1 article reviews
orion ii microplate luminometer - by Bioz Stars, 2026-09
90/100 stars

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GEF Assay:

Article Title: TRIM23 mediates cGAS-induced autophagy in anti-HSV defense.
Article Snippet: Subsequently, the GTPase activity was determined by a GTPase-Glo assay (V7681; Promega) following themanufacturer’s recommendations. .. In brief, GEF assay buffer (containing 1mM DTT and 0.1mg/mL cardiolipin) was added to the beads, and incubation with 2X GTP solution was carried out at 37 °C for 16 h. Following this, the GTPase-Glo reagent and the detection reagent were added, and luminescence detection was performed using an Orion II microplate Luminometer (Berthold Detection Systems). ..

Article Title: TRIM23 mediates cGAS-induced autophagy in anti-HSV defense
Article Snippet: Subsequently, the GTPase activity was determined by a GTPase-Glo assay (V7681; Promega) following the manufacturer’s recommendations. .. In brief, GEF assay buffer (containing 1 mM DTT and 0.1 mg/mL cardiolipin) was added to the beads, and incubation with 2X GTP solution was carried out at 37 °C for 16 h. Following this, the GTPase-Glo reagent and the detection reagent were added, and luminescence detection was performed using an Orion II microplate Luminometer (Berthold Detection Systems). ..

Incubation:

Article Title: TRIM23 mediates cGAS-induced autophagy in anti-HSV defense.
Article Snippet: Subsequently, the GTPase activity was determined by a GTPase-Glo assay (V7681; Promega) following themanufacturer’s recommendations. .. In brief, GEF assay buffer (containing 1mM DTT and 0.1mg/mL cardiolipin) was added to the beads, and incubation with 2X GTP solution was carried out at 37 °C for 16 h. Following this, the GTPase-Glo reagent and the detection reagent were added, and luminescence detection was performed using an Orion II microplate Luminometer (Berthold Detection Systems). ..

Article Title: TRIM23 mediates cGAS-induced autophagy in anti-HSV defense
Article Snippet: Subsequently, the GTPase activity was determined by a GTPase-Glo assay (V7681; Promega) following the manufacturer’s recommendations. .. In brief, GEF assay buffer (containing 1 mM DTT and 0.1 mg/mL cardiolipin) was added to the beads, and incubation with 2X GTP solution was carried out at 37 °C for 16 h. Following this, the GTPase-Glo reagent and the detection reagent were added, and luminescence detection was performed using an Orion II microplate Luminometer (Berthold Detection Systems). ..

other:

Article Title: Structures of TGF-β with betaglycan and signaling receptors reveal mechanisms of complex assembly and signaling.
Article Snippet: Equilibrium binding assays were performed by injecting the analytes in 10mM HEPES, 150mM NaCl, 0.1% surfactant P20 (Pierce) at pH 7.4 (HBS-EP buffer) at a rate of 10μl min−1 for 720 s, followed by a dissociation period of 600 s. Regeneration of the surface was achieved by a 10 s injection at 100μl min−1 of 4M guanidine hydrochloride in 4mM HCl solution.

Article Title: Exposure to fungi, bacteria, and endotoxin in a museum, with staff reporting work-related symptoms.
Article Snippet: The HL-60 cells were exposed to the dust suspensions and references, after which the production of reactive oxygen species (ROS) was measured by a luminoldependent chemiluminometric assay using a thermostatic (37 �C) ORION II Microplate Luminometer (Berthold Detection Systems) (Madsen et al. 2020, Timm et al. 2006).

Lysis:

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response 1.
Article Snippet: .. 275 Twenty microliters of cell lysis supernatant were combined with 100 μL of assay buffer (15 mM 276 potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO4, 4 mM EDTA, 2 mM 277 adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was 278 measured on an Orion II microplate luminometer (Berthold Detection Systems, Oak Ridge, TN, 279 USA). ..

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response one
Article Snippet: .. Twenty microliters of cell lysis supernatant were combined with 100 μl of assay buffer (15 mM potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO 4 , 4 mM EDTA, 2 mM adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was measured on an Orion II microplate luminometer (Berthold Detection Systems). ..

Luciferase:

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response 1.
Article Snippet: .. 275 Twenty microliters of cell lysis supernatant were combined with 100 μL of assay buffer (15 mM 276 potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO4, 4 mM EDTA, 2 mM 277 adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was 278 measured on an Orion II microplate luminometer (Berthold Detection Systems, Oak Ridge, TN, 279 USA). ..

Article Title: miR‐486‐5p Inhibits eNOS and Angiogenesis in Cultured Endothelial Cells by Targeting MAML3
Article Snippet: The pEZX‐MT06‐eNOS 3′ UTR‐f/rLuc or mutant eNOS 3′ UTR‐f/rLuc vectors (50 ng) were transfected into HUVECs in a 96‐well plate with Lipofectamine 3000 alone, or with 10 nM miR‐486‐5p mirVana mimic (Thermo Fisher). .. Luciferase activity was determined after 24 h using a Dual Luminescence assay kit (Genecopeia) by an Orion II microplate luminometer (Berthold Detection Systems, Pforzheim, Germany). .. Cell proliferation was evaluated by measuring BrdU incorporation during DNA synthesis (Cell Proliferation ELISA, BrdU (colorimetric), Roche, Laval, QC, Canada) as per the manufacturer's protocol.

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response one
Article Snippet: .. Twenty microliters of cell lysis supernatant were combined with 100 μl of assay buffer (15 mM potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO 4 , 4 mM EDTA, 2 mM adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was measured on an Orion II microplate luminometer (Berthold Detection Systems). ..

Activity Assay:

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response 1.
Article Snippet: .. 275 Twenty microliters of cell lysis supernatant were combined with 100 μL of assay buffer (15 mM 276 potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO4, 4 mM EDTA, 2 mM 277 adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was 278 measured on an Orion II microplate luminometer (Berthold Detection Systems, Oak Ridge, TN, 279 USA). ..

Article Title: miR‐486‐5p Inhibits eNOS and Angiogenesis in Cultured Endothelial Cells by Targeting MAML3
Article Snippet: The pEZX‐MT06‐eNOS 3′ UTR‐f/rLuc or mutant eNOS 3′ UTR‐f/rLuc vectors (50 ng) were transfected into HUVECs in a 96‐well plate with Lipofectamine 3000 alone, or with 10 nM miR‐486‐5p mirVana mimic (Thermo Fisher). .. Luciferase activity was determined after 24 h using a Dual Luminescence assay kit (Genecopeia) by an Orion II microplate luminometer (Berthold Detection Systems, Pforzheim, Germany). .. Cell proliferation was evaluated by measuring BrdU incorporation during DNA synthesis (Cell Proliferation ELISA, BrdU (colorimetric), Roche, Laval, QC, Canada) as per the manufacturer's protocol.

Article Title: Gonadotropin-releasing hormone regulates transcription of the inhibin B co-receptor, TGFBR3L, via early growth response one
Article Snippet: .. Twenty microliters of cell lysis supernatant were combined with 100 μl of assay buffer (15 mM potassium phosphate [pH 7.8], 25 mM glycylglycine, 15 mM MgSO 4 , 4 mM EDTA, 2 mM adenosine triphosphate, 1 mM dithiothreitol, 0.04 mM D-luciferin), and luciferase activity was measured on an Orion II microplate luminometer (Berthold Detection Systems). ..

Luminescence Assay:

Article Title: miR‐486‐5p Inhibits eNOS and Angiogenesis in Cultured Endothelial Cells by Targeting MAML3
Article Snippet: The pEZX‐MT06‐eNOS 3′ UTR‐f/rLuc or mutant eNOS 3′ UTR‐f/rLuc vectors (50 ng) were transfected into HUVECs in a 96‐well plate with Lipofectamine 3000 alone, or with 10 nM miR‐486‐5p mirVana mimic (Thermo Fisher). .. Luciferase activity was determined after 24 h using a Dual Luminescence assay kit (Genecopeia) by an Orion II microplate luminometer (Berthold Detection Systems, Pforzheim, Germany). .. Cell proliferation was evaluated by measuring BrdU incorporation during DNA synthesis (Cell Proliferation ELISA, BrdU (colorimetric), Roche, Laval, QC, Canada) as per the manufacturer's protocol.



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